SOME NEW DATA ABOUT ANTIVIRAL AND RELATED ACTIVITIES OF SEABUCKTHORN PRINCIPALS AND THE PROSPECTS OF THEIR USE

SHIPULINA L.D., All-Russian Research Institute of Medicinal and Aromatic Plants, Moscow, Russia

 

The problem of treatment of diseases caused by various viruses, belong today to a number most urgent tasks. The creation of antiviral remedies is one of the most complex tasks of chemotherapy of infections, in particular due to the RNA and DNA-containing viruses belong to obligatory intracellular parasites. During reduplication the viruses basically use the device of the cell biosynthetic device of cells of macroorganism, modifying in some manner it, therefore it is extremely difficult to find out selective remedies, killing viruses not damaging the host cells. Among the viral infectious influenza occupies one of the first places in number of people involved in an epidemic process. For a long time as the most effective method of struggle with virus infections is considered vaccinoprophylactic. However in case of influenza virus the efficiency of these measures is limited owing to unusual antigenic variability resulting in appearance of new virus strains capable to overcome the immunity produced to previous strains. Therefore chemoprophylactic and chemotherapy of virus infections (influenza and acute respiratory viral infections, including adenoviral infections) are rather urgent directions. Basic chemotherapeutic methods of treatment of influenza nowadays are derivatives of amantadin, including remantadin. The negative property of these preparations is the presence therapeutic effect only at early stages of illness in case of Influenza A and absence of efficiency at Influenza . Besides that, their wide application results in appearance of remantadin resistant strains of virus of Influenza A. The importance of Herpes viral infections as the problems for public health services grows worldwide. According to the WHO human virus Herpes Simplex strains infect 60-95 % of the population, both in advanced, and in the developing countries, wile Herpes viral infections occupy the second place (15.8 %) after influenza (35.8 %) as the reason cause of mortality from virus infections. Herpes viral infection varies from rash form on a skin and mucosa up to encephalitis, striking vital - important of internal organs. Herpes genitalis in a number of countries nowadays is attributed to venereal diseases. The infection in neonatal and perinatal periods results in high indexes of mortality of newborn (till 50-80 %), and in survived infants to serious consequences. Herpes viral infections proceed as hardest complications for the patients with the lowered immunity, including for post-transplantaion and oncological patients, at the HIV-infection etc. The basic difficulty in treatment Herpes viral diseases is the fact, that the Herpes viruses contrary to several other viruses, are characterized by ability to persistency in the organism and reactivation under influence of the various adverse factors with development of relapses of disease. This circumstance emphasizes necessity of creation rather harmless antiviral preparations, suitable for treatment during long and repeated application. The absence of effective preparations of etiotropic action at the HIV-infection, hardest disease of the present time, is the sufficient basis for search of medical remedies and, first of all, among natural substances.

The basic task of the present work consists in an establishment of the specific chemotherapeutic action of Hiporamin on various experimental models of virus infections. Hiporamin is the original drug produced from Hippophae rhamnoides, which is the dry purified extract of the polyphenol complex of gallo-ellagi-tannins. Biologically active components of Hiporamin are hydrolizable tannins: (strictinin, isostrictinin, casuarinin, hipophaenin D and the substance of nontannin nature - quebrachitol. The study is carried out in respect of various viral strains, including epidemic viruses of Influenza A and B, adenoviruses, herpes and HIV-infection. Besides that there is investigated an antimicrobial spectrum of Hiporamin in respect of pathogenic bacteria and fungi.

Thus, experimental data obtained have shown, that Hiporamin possesses a unique biological properties combining antiviral activity in respect of wide spectrum of pathogenic DNA and RNA containing viruses (Influenza A and B, Herpes, adenoviruses, HIV etc.), pathogenic micro-organisms, and at the same time exhibits interferon inducting property. Just as a number of cells (FEC, MDCK, Vero Hep-2) are resistant to Hiporamin at the concentration exceeding many times its therapeutic doses, while the drug possesses a high CTI. Results produced have been put into the basis of working out a new efficient drug in various pharmaceutical forms, which were clinically tried at viral infections in a number of medical establishments.

tannin nature - quebrachitol. The study is carried out in respect of various viral strains, including epidemic viruses of Influenza A and B, adenoviruses, herpes and HIV-infection. Besides that there is investigated an antimicrobial spectrum of Hiporamin in respect of pathogenic bacteria and fungi.

Thus, experimental data obtained have shown, that Hiporamin possesses a unique biological properties combining antiviral activity in respect of wide spectrum of pathogenic DNA and RNA containing viruses (Influenza A and B, Herpes, adenoviruses, HIV etc.), pathogenic micro-organisms, and at the same time exhibits interferon inducting property. Just as a number of cells (FEC, MDCK, Vero Hep-2) are resistant to Hiporamin at the concentration exceeding many times its therapeutic doses, while the drug possesses a high CTI. Results produced have been put into the basis of working out a new efficient drug in various pharmaceutical forms, which were clinically tried at viral infections in a number of medical establishments.